y: In this study,  Global Burden of Diseases (GBD) methodology for measuring the burden of chronic diseases in the main two entities of Palestinian territories was adapted, using Disability-Adjusted Life Years (DALYs) measurement. The measurement tools and their standards templates were specially designed for assessing the burden of disease, developed by the World Health Organization (WHO) to estimate and quantify the burden of chronic diseases. Thestudy demonstrates that heart diseases followed with cancer remains the major chronic diseases burden in the Palestinian healthcare sector in the Palestinian territories in both Gaza strip and West bank, and recommends that  further studies using more recent data are important and needed